1.1This standard is designed for use with a mechanized device (the Wiperator; Appendix X1) to test pre-wetted towelettes.

1.2Two species of vegetative bacteria, one Gram-positive coccus (Staphylococcus aureus) and one Gram-negative bacillus (Acinetobacter baumannii), representing important nosocomial pathogens, are used to separately contaminate disks of magnetized and brushed stainless steel in order to test the towelettes for their relative ability to:

1.2.1Decontaminate non-porous environmental surfaces experimentally-contaminated with vegetative bacteria; and

1.2.2Transfer any acquired bacterial contamination on the towelettes to clean surfaces.

1.3This test method is not meant for use with towelettes for decontamination of skin.

1.4The values stated in SI units are to be regarded as the standard. The values given in parentheses, if any, are for information only.

1.5This test method should be performed by persons with training in microbiology in facilities designed and equipped for work with infectious agents at the appropriate biosafety level.

1.6It is the responsibility of the investigator to determine whether Good Laboratory Practice (GLP) regulations are required and to follow them where appropriate.

1.7This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.